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Morphological and Biochemical Assessment of Papain-Induced Emphysema
Kaye H. Kilburn, MD;
Anthony R. Dowell, MD;
Philip C. Pratt, MD
Arch Intern Med. 1971;127(5):884-890.
Abstract
A model for emphysema was produced in Syrian hamsters by one intratracheal injection of 1 mg of purified papain in saline solution. Papain produced pulmonary edema and hemorrhage initially but after four days collagen was lost from alveolar walls. Departitioning of alveoli occurred despite normal synthesis of lecithin and, probably, increased synthesis of protein. Total lung protein, collagen, and elastin did not change. Alveolar basement membrance connective tissue, particularly collagen, was digested but repair was stimulated as judged from cellular changes and protein synthesis rates. Two interpretations are possible: either collagen is redistributed in alveoli by digestion and synthesis with loss of interstitial connective tissue or the collagen content in alveolar walls is so small a proportion of total lung collagen that its disappearance was not measured.
Author Affiliations
Durham, NC
From Duke University Medical Center and the Durham Veterans Administration Hospital, Durham, NC.
Footnotes
Received for publication Oct 21, 1970; accepted Feb 15, 1971.
Read in part before the Tenth Annual Biology Symposium on Pollution and Lung Biochemistry, Richland, Wash, June 5, 1970, jointly sponsored by the Battelle Memorial Institute-Pacific Northwest Laboratories, National Air Pollution Control Administration, National Institute of Environmental Health Sciences, and the US Atomic Energy Commission.
Reprint requests to Department of Medicine, Duke University Medical Center, Durham, NC 27706 (Dr. Kilburn).
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