 |
|
The Origin of Pulmonary Alveolar MacrophagesStudies of Stem Cells Using the Es-2 Marker of Mice
Mary-Ann Brunstetter;
Jeanne A. Hardie;
Robert Schiff, PhD;
Jerry P. Lewis, MD;
Carroll E. Cross, MD
Arch Intern Med. 1971;127(6):1064-1068.
Abstract
Pulmonary alveolar macrophages (PAMs) constitute a major lung cellular defense mechanism. There is still considerable controversy concerning the cellular precursors of the resident PAM population. Genetically related (RF/AI( + ) and RF/AI(-) substrains of mice were used to investigate the possibility of PAM origin from marrow stem cells. The RF/AI( +) mice carry a nonspecific readily identifiable prealbumin esterase marker, Es-2. Reciprocal chimerism was produced by administering a lethal dose of x-irradiation to (1) RF/AI(-) mice and subsequently injecting them with RF/AI( + ) bone marrow, and (2) RF/ Al( +) mice and subsequently injecting them with RF/AI(-) bone marrow. Eight weeks after irradiation pulmonary macrophages were obtained by bronchial lavage. PAMs from RF/AI(-) animals transplanted with RF/AI( +) bone marrow showed the prealbumin esterase marker, Es-2, while PAMs from the RF/AI( + ) animals given RF/AI(-) bone marrow had all but lost their Es-2 marker band, which confirms the marrow origin of PAMs.
Author Affiliations
Davis, Calif; Boston; Davis, Calif
From the Department of Medicine, School of Medicine, University of California, Davis (Misses Brunstetter and Hardie and Drs. Lewis and Cross) and the Department of Anatomy, Tufts University School of Medicine, Boston (Dr. Schiff).
Footnotes
Received for publication Oct 20, 1970; accepted March 11, 1971.
Read in part before the Tenth Annual Hanford Biology Symposium on Pollution and Lung Biochemistry, Richland, Wash, June 3, 1970, jointly sponsored by the Battelle Memorial Institute-Pacific Northwest Laboratories, National Air Pollution Control Administration, National Institute of Environmental Health Sciences, and the US Atomic Energy Commission.
Read in part before the annual meeting of the American Association of Clinical Research, May 3, 1970, Atlantic City, NJ.
Reprint requests to Department of Medicine, School of Medicine, University of California, Davis, Calif 95616 (Dr. Cross).
 CiteULike  Connotea  Delicious  Digg  Facebook  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Differentiation of C2D Macrophage Cells after Adoptive Transfer
Potts et al.
CVI 2008;15:243-252.
ABSTRACT
| FULL TEXT
Prevention of atherosclerosis in apolipoprotein E-deficient mice by bone marrow transplantation
Linton et al.
Science 1995;267:1034-1037.
ABSTRACT
Direct evidence for a bone marrow origin of the alveolar macrophage in man
Thomas et al.
Science 1976;192:1016-1018.
ABSTRACT
|
