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Further Studies of the Enzyme Composition of Mutant Cells in X-Linked Uric Aciduria
Lawrence Sweetman, PhD;
William L. Nyhan, MD, PhD
Arch Intern Med. 1972;130(2):214-220.
Abstract
Assay procedures have been developed for the determination of hypoxanthine guanine phosphoribosyltransferase (HGPRT) and adenine phosphoribosyltransferase (APRT) activity in erythrocytes and for HGPRT and inosinic acid dehydrogenase activity in fibroblasts. In normal erythrocytes the mean activity for HGPRT using hypoxanthine as substrate was 39.09 mµmols/hr/µl of cells. The mean value for patients with the Lesch-Nyhan syndrome was 0.003 mµmols/hr/µl. Nearly all of the obligate heterozygotes studied had normal levels of HGPRT activity in both erythrocytes and fibroblasts. The normal erythrocyte mean level of APRT activity was 23.56 mµmols/hr/µl. All subjects with complete or partial deficiency of HGPRT and heterozygotes had elevated APRT activity. There were no differences among the inosinic acid dehydrogenase (IMPDH) activities of fibroblasts from subjects who were normal, HGPRT-deficient, or hyperuricemic without HGPRT deficiency.
Author Affiliations
Miami, Fla; La Jolla, Calif
From the Department of Biochemistry, University of Miami School of Medicine, Miami, Fla (Dr. Sweetman), and the Department of Pediatrics, University of California, San Diego School of Medicine, La Jolla (Dr. Nyhan). Dr. Sweetman is now with the Department of Pediatrics, University of California, San Diego School of Medicine, La Jolla.
Footnotes
Received for publication Jan 5, 1972; accepted March 10.
Reprint requests to Department of Pediatrics, University of California, San Diego School of Medicine, La Jolla, Calif 92037 (Dr. Sweetman).
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