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Some Observations in Vitro

R. SHIHMAN CHANG, M.D., D.Sc.

Arch Intern Med. 1962;110(5):563-568.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

This report describes experiments designed to study changes in metabolic patterns associated with the senescence of human amnion cells in vitro. Senescence has been defined as the increase in the chronological age of a population of cultured cells which are predominantly nondividing. This definition is based on the concept formulated by Comfort and Shock.¹ The general scheme of these experiments consists of the determination of radioactivity in various cell components obtained from cultures previously labeled with C14 substrates.

Materials and Methods

Amnion Culture.—

Cell suspensions were prepared from amniotic membranes carefully dissected from placentae obtained at normal full-term deliveries.² Approximately 5 million cells suspended in 10 ml. of nutrient medium, consisting of 10% human and 5% calf or 20% horse serum in Eagle's basal medium,³ were implanted in milk dilution bottles of 200 ml. capacity. The media were renewed on the 4th and 7th days. On the . . . [Full Text PDF of this Article]

Author Affiliations
BOSTON

Department of Microbiology, Harvard School of Public Health.

Footnotes
Supported by research grants (E553C9 and GMK3-15,131-C4) from the U.S. Public Health Service.

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