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STANLEY M. LEVENSON, M.D.; BUD TENNANT, D.V.M.; ERVING GEEVER, M.D., Ph.D.; LT. ROGER LAUNDY, V.C.; FLOYD DAFT, Ph.D.

Arch Intern Med. 1962;110(5):693-702.

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Introduction

In the course of some studies of the effects of injury and infection on the metabolism of ascorbic acid, it became important for us to know how the microorganisms present in ordinary healthy laboratory animals influence the metabolism of this vitamin. To this end, we followed the responses of germ-free and purposely contaminated guinea pigs to a scorbutigenic diet.

Methods

Walter Reed Strain^* guinea pigs were used. Pregnant animals (anesthetized with pentobarbital sodium) were delivered aseptically at term by cesarean section using a special Reyniers Germ-free Operating Unit.¹ The newborn guinea pigs were transferred immediately following birth into sterile Reyniers Series 400 Rearing Units. These stainless steel tanks may be maintained under germ-free conditions for periods of one or more years. Air is sterilized by passage through fiber glass filters. Food, water and other supplies are generally sterilized by steam under pressure and entered into the germ-free chamber . . . [Full Text PDF of this Article]

Author Affiliations
NEW YORK

From the Departments of Germfree Research (Dr. Tennant and Lt. Laundy) and Surgical Pathology and Metabolism (Dr. Geever), The Division of Basic Surgical Research, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, 12, D.C. and the Departments of Surgery (Dr. Levenson and Dr. Daft) and Biochemistry (Dr. Daft), Albert Einstein College of Medicine, Yeshiva University, New York 61, New York.

Footnotes
This work was supported in part by a Grant No. DA-49-193-61-G27MD, to the Albert Einstein College of Medicine by the Research and Development Command of the U.S. Army Medical Service and Grant No. A-5664 to the Albert Einstein College of Medicine by the National Institutes of Health.

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