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The Probable Metabolically Active Form of Vitamin D3: Its Identification and Subcellular Site of Action

H. F. DeLuca, PhD

Arch Intern Med. 1969;124(4):442-450.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

It has been assumed from the early work of Askew et al¹ and Windaus and co-workers^2,3 that vitamins D₂ and D₃ act directly in the organism without molecular change. Experiments by Kodicek^4,5 first demonstrated that vitamin D is in fact metabolized to biologically inactive "break down products" and lent further support to this assumption. In their hands, no evidence could be obtained for a biologically active metabolite of the vitamin. Clearly, their experiments were hindered by the low specific activity of vitamin D₂ labeled with radioactive carbon (¹⁴C) and by inadequate chromatographic methods so as to preclude the detection of a major biologically active metabolite. With the development of new and powerful chromatographic techniques ^6,7 and the synthesis of a radioactive vitamin D preparation of high enough specific activity ^8-10 to allow experiments with physiologic doses of the vitamin, the question of vitamin . . . [Full Text PDF of this Article]

Author Affiliations
Madison, Wis

From the Biochemistry Department, University of Wisconsin, Madison. Dr. DeLuca is a Steenbock research professor.

Footnotes
Received for publication June 11, 1969; accepted July 16.

Read before the Conference on Divalent Ion Metabolism and Osteodystrophy in Chronic Renal Failure, Santa Barbara, Calif, Nov 18, 1968.

Reprint requests to Biochemistry Department, University of Wisconsin, Madison 53706 (Dr. DeLuca).

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