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Kenneth M. Pruitt, PhD; Maw Jian Cherng, MS; Hugh L. Spitzer

Arch Intern Med. 1971;127(3):390-394.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Surfactant has been prepared by ultracentrifugation and density gradient centrifugation from pig lung lavage. The product is a lipoprotein, free of contamination by serum proteins, and containing 4 mg of phospholipid per milligram of protein. The lipid portion is 60% dipalmitoyl-lecithin plus small amounts of other phospholipids. The surfactant exhibits ultraviolet fluorescence. Excitation at 260±10 mµ gives a single emission maximum at 320±10 mµ. Anilinonaphthalene sulfonate binds to surfactant and gives an enhanced fluorescence peak at 465±10 mµ when excited at 375 ± 10 mµ.

Mammalian lungs are lined by a surface active material which may help to prevent alveolar collapse at small lung volumes. Reduced quantities of this material are associated with the lung collapse observed in hyaline membrane disease.¹ Although the surface active properties of alveolar wash can be duplicated by dipalmitoyl-lecithin alone,² it does not necessarily follow that pure dipalmitoyl-lecithin lines the alveolar surface. Indeed, . . . [Full Text PDF of this Article]

Author Affiliations
Birmingham, Ala

From the Department of Medicine and Laboratory of Molecular Biology, University of Alabama Medical Center, Birmingham. Dr. Pruitt is now on sabbatical leave with the Department of Pharmacology, Hershey Medical Center, Hershey, Penn.

Footnotes
Received for publication Oct 21, 1970; accepted Jan 11.

Read in part before the Tenth Annual Hanford Biology Symposium on Pollution and Lung Biochemistry, Richland, Wash, June 3, 1970, jointly sponsored by the Battelle Memorial Institute-Pacific Northwest Laboratories, National Air Pollution Control Administration, National Institute of Environmental Health Sciences, and the US Atomic Energy Commission.

Reprint requests to Pulmonary Division, University of Alabama, Birmingham, Ala 35233 (Mr. Spitzer).

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