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Its Use in Testing for Sensitivity to Antibiotics

JOSEPH FELSEN, M.D.; ALFRED J. WEIL, M.D.

AMA Arch Intern Med. 1951;88(3):406-408.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The divided culture plate¹ was devised principally as a practical aid in the bacteriologic diagnosis of enteric infection and for antibiotic studies. It consists of the standard 94-mm. Petri-dish bottom, which is separated into quarters by two smooth bisecting ridges of glass 5.5 mm. in height. The inside height of the bottom is 13 to 15 mm., and the covers are identical with those of ordinary Petri dishes. The quadrants are marked I to IV for easy identification. In studies on enteric pathogens, the suspected material (feces or, preferably, exudate obtained by mucosal crypt aspiration²) is seeded on the quadrants, each of which contains a different culture medium chosen for its selectivity (e. g., Endo's [fuchsin sulfite agar], S. S. [Salmonella-Shigella thiosulfate-citrate bile agar], desoxycholate citrate agar, and MacConkey's [bile-salt agar] or E. M. B. [eosin methylthionine chloride agar]). The plates are incubated overnight and suspicious colonies picked . . . [Full Text PDF of this Article]

Author Affiliations
NEW YORK

From the Department of Laboratories and Medical Research, The Bronx Hospital.

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