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STUDIES ON STREPTOCOCCAL HYALURONIDASE AND ANTIHYALURONIDASE
JOSEPH M. DI CAPRIO, M.D.;
LOWELL A. RANTZ, M.D.;
ELIZABETH RANDALL, M.A.
AMA Arch Intern Med. 1952;89(3):374-386.
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THERE have been numerous reports in recent years dealing with hyaluronic acid, hyaluronidases, and hyaluronidase inhibitors. The relationship of these substances to Group A hemolytic streptococcal infections and rheumatic fever has been the chief interest of this laboratory. The present study was undertaken in an effort to gain additional information regarding this aspect of the problem.
The mucin-clot-prevention method was used to measure both streptococcal hyaluronidase and streptococcal antihyaluronidase. This method has been described by McClean1 and was based upon the observations of Meyer and Palmer,2 Seastone,3 and Robertson, Ropes, and Bauer.4 The basis of the test was the observation that hyaluronic acid, when mixed with protein and acidified, produced a mucin clot and that this reaction was inhibited by the presence of hyaluronidase. This test has been employed by subsequent investigators5 and modified by us as described below.
METHODS
1. Preparation of Hyaluronic Acid.
—Human umbilical cords6 were used
. . . [Full Text PDF of this Article]
Author Affiliations
SAN FRANCISCO
From the Department of Medicine, Stanford University School of Medicine, San Francisco.
Footnotes
This investigation was performed, in part, under contract with the Commission on Acute Respiratory Diseases, Armed Forces Epidemiological Board; aided by a grant from the Life Insurance Medical Research Fund, and supported in part by a research grant from the National Heart Institute of the National Institutes of Health, United States Public Health Service.
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