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Study on Healthy and Diseased Subjects

SMITH FREEMAN, M.D., Ph.D.; J. X. WHEELER, B.S.; H. W. HOEGEMEIER, M.D.

AMA Arch Intern Med. 1956;97(1):45-50.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

One of the earliest attempts to estimate chemically the concentration of adrenal cortex steroids in blood was made by Hemphill and Reiss, who measured the total reducing ability of blood-lipid extracts by means of the Hagedorn-Jensen blood sugar method.¹ In 1948, Corcoran and Page² applied the procedure for the measurement of formaldehydegenic steroids to extracts of blood plasma. Recent chemical methods have provided a more specific means for the measurement of adrenocortical substances with a 17-hydroxy-ketol side-chain, such as occur in compounds E (cortisone), F (hydrocortisone), and S.

In 1952, Nelson and Samuels ³ reported a method for the direct quantitation of 17-hydroxy-adrenocortical steroids. According to their procedure, the blood extract was purified by solvent partition and chromatography on a synthetic magnesium silicate (Florisil) column. The 17-hydroxy blood corticosteroids were estimated by a micromodification of the phenylhydrazine reaction as introduced by Porter and Silber,⁴ which is stated . . . [Full Text PDF of this Article]

Author Affiliations
Chicago

From the Veterans Administration Hospital, Hines, Ill., and the Department of Biochemistry, Northwestern University Medical School, Chicago.

Footnotes
Submitted for publication May 18, 1955.

This study was partially supported by a research grant, No. C-1210, from the National Cancer Institute of the National Institutes of Health, United States Public Health Service.

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